Pdf infection of soybean with soybean mosaic virus increases. In wisconsin in 20 numerous products were evaluated for white mold control in soybean. Fine mapping and candidate gene discovery of the soybean mosaic virus resistance gene, rsv4 m. Symptoms on soybean include stunting, and leaf puckering, rugosity, and yellowing. Mosaic viruses are mostly spread by insects, especially aphids and leafhoppers. Smv is a member of the genus potyvirus berger et al. Soybean mosaic virus smv soybean mosaic virus is transmitted through seed at rates ranging from 0 to 5% in some commercial varieties, and by several species of aphids, including soybean aphid. It infects mainly plants belonging to the family fabaceae but has also been found infecting other economically important crops. Agronomic impact our experience over the past few years. Soybean mosaic virus smv is one of the most devastating pathogens that cost huge economic losses in soybean production worldwide. Breakdown of resistance in sweet pepper against pepper yellow. Viruses are prevalent in cultivated crops, weeds, and plants in natural ecosystems.
The disease may be difficult to detect as symptoms look similar to several nutrient deficiencies. Mosaic virus infects roses, tulips, beets, plums, tobacco, beans, peppers, and cucumbers. Leaves may appear as a mottled mixture of dark green, light green, and yellow with a distorted shape. Seo jk1, ohshima k, lee hg, son m, choi hs, lee sh, sohn sh, kim kh. Soybean mosaic virus and alfalfa mosaic virus badger. Enhancement by soybean mosaic virus of bean pod mottle virus. The virus particle is a long, flexuous rod containing a single strand of rna. While these and other cucurbits can be stricken, cucumber mosaic virus cmv regularly attacks a wide variety of garden vegetables and ornamentals as well as common weeds. Fungicide efficacy testing results for mycosphaerella blight ascochyta from carrington rec. Tolin abstract soybean mosaic virus smv is a prevalent virus infecting soybean glycine max l. Symptoms of smv vary with variety, virus strain, environment, and age of plant at infection.
Purification of soybean mosaic virus for antiserum production. Control bean yellow mosaic is not seedtransmitted, thus roguing is of no value. Dec 10, 2009 the study was carried out to determine the comparative pathogenic response of six cultivars of soybean. Check the soybean seeds october, 1997 soybean seed coat mottling june, 1998 discolored soybean seeds october, 1998.
Variety resistance reported, but commercial varieties are not rated for reaction to smv. Goodman graduate research assistant and associate professor, respectively, department of plant pathology and international soybean program. An association of bean yellow mosaic virus bymv, genus potyvirus, with yellow mosaic leaf symptoms on soybean in argentina was determined by enzymelinked immunosorbent assay, a result. This concerns, for example, bean pod mottle virus, cucumber mosaic, peanut stunt and soybean stunt viruses, tobacco ringspot virus and tobacco streak virus frison et al. The study was carried out to determine the comparative pathogenic response of six cultivars of soybean. Engineering a decoy substrate in soybean to enable recognition. Mixed infections of bean yellow mosaic virus and soybean mosaic virus are common in soybeans. Aphids, leafhoppers, whiteflies and cucumber beetles are common garden pests that can transmit this disease. Field incidences of smv correlate with flights of the soybean aphid burrows et al. Bean yellow mosaic virus is a plant pathogenic virus in the genus potyvirus and the virus family potyviridae. Seed resistant isolines as fundamental controls, or monitor. Soybean mosaic virus smv is a member of the potyvirus group hill, 1999. This virus was first identified in iowa and the united states in 1967.
Apple mosaic virus apmv is a plant pathogenic virus of the family bromoviridae. Cucumber mosaic disease was first reported in north america around 1900 and has since spread worldwide. The disease itself has several synonyms including mild apple mosaic virus, hop virus, rose mosaic virus, and european plum line patten virus. The sample came back strongly positive for tobacco streak virus tsv. The incidence of smv infection in soybean fields has been much lower than that reported for bpmv. Managing white mold in soybean wisconsin field crops. Soybean mosaic virus and bean pod mottle virus seed infected by soybean mosaic virus left and by bean pod mottle virus right. General symptoms of smv and amv infection are plant stunting, leaf distortion, rugosity and mottling, reduced pod numbers and seed discoloration. Molecular variability and genetic structure of the. Trifoliate leaves show distinct mosaic and mottling symptoms with light and dark green areas that later can become raised or blistered along the. Tgx 184418e, tgx 14482e, tgx 19108f, tgx 10192en, tgx 19108f and tgx 18764e to single and mixed infections with cowpea mottle virus and cucumber mosaic virus. Soybean mosaic virus and bean pod mottle virus act synergistically. Bean pod mottle virus bpmv is a virus disease spread primarily by the bean leaf beetle, cerotoma trifurcata. Again, there is no treating mosaic infections in beans.
Sep 27, 20 after eliminating all of the known causes, a sample was screened for several common bean viruses. Effect of single and double infections of soybean mosaic virus and bean pod mottle virus on soybean yield and seed characters. Identified differential responses of soybean germplasm to bean pod mottle virus. It has a positive sense single stranded rna ssrna genome of approximately 10 kb in length, a genome linked viral protein vpg covalently bound to the 5. Detection, understanding and control of soybean mosaic virus. Soybean mosaic virus smv is a member of the plant virus family potyvirideae and is the cause of soybean mosaic disease, which occurs in all soybean production areas of the world. Fungicide recommendations are developed by the ncera7 national soybean disease committee, and recommendations are based on replicated research data collected from university trials. Viruses added included cmv, alfalfa mosaic virus amv, and two potyviruses, bean common mosaic virus and bean yellow mosaic virus. Soybean mosaic virus differs from bean yellow mosaicand bean common mosaicviruses in that i it fails to cause systemic symptoms in phaseolus vulgaris cultivars excepting double white princess, ii bean common mosaic virus does not infect soybean systemically, and iii bean yellow mosaic virus infects vicia faba. Infection by both viruses induce severe symptoms and terminal death may occur.
Role of soybean mosaic virus encoded proteins in seed and aphid transmission in soybean. Due to the duplicated genome, clustered and highly homologous nature of r genes, as well as recalcitrant to transformation, soybean disease resistance studies is largely lagging compared with other diploid crops. The disease caused by this potyvirus was controlled after the introduction of. Soybean mosaic virus avirulence determinants corresponding to the rsv genes. Entomology image gallery plant diseases and damage soybean soybean mosaic virus. Smv is much more studied than bean pod mottle virus. Soybean mosaic virus helper componentprotease alters leaf. A synonym of the virus is subterranean clover red leaf virus. After eliminating all of the known causes, a sample was screened for several common bean viruses. Mixed infections of bean yellow mosai c virus and soybean mosaic virus are common in soybeans. The most common virus of soybean around the world is soybean mosaic virus smv.
Differentiation of soybean mosaic virus isolates by onedimensional trypsin peptide maps immunoblotted with monoclonal antibodies. Soil, seed, starter pots and containers can be infected and pass the virus to the plant. Pdf detection, understanding and controlof soybean mosaic virus. African cassava mosaic virus acmv, east african cassava mosaic virus eacmv, and south african cassava mosaic virus sacmv are distinct species of circular singlestranded dna viruses that are whitefly transmitted and primarily infect cassava plants. The soybean plants with pods showing necrotic spots were noticed in lateplanted soybeans. Soybean mosaic virus smv is a prevalent pathogen that causes significant yield reduction in soybean production worldwide. Infectious virus was not detected in testas was detected in 100% of the flowers, immature seeds, and green pods that from mature midwest seeds by a local lesion index, but the same testas were tested but not in any of the dry pods. Mosaic virus control mosaic virus is a viral disease found in many gardens throughout the united states. Smv is spread by several aphid species, and the major source of infection is soybean, for this virus is commonly seedborne in that crop. We discovered a soybean mosaic virus smv variant 42781 that caused systemic infections in nicotiana benthamiana plants, resulting in stem stunting and leaf shriveling. Cole trebesch said he follows university threshold recommendations to control soybean aphids in his brown county, minnesota, fields. Soybean mosaic virus but not in those of merit seeds.
Pea seedborne mosaic virus psbmv in field peas and lentils pp1704 2014. Coinfection of both viruses increased the yield loss to as high as 86% and increase the rate of. Like other members of the potyvirus genus, it is a monopartite strand of positivesense, singlestranded rna surrounded by a capsid made for a single viral encoded protein. In nebraska, smv is not as common as bpmv based on field surveys conducted from the 2000 to 2002 growing seasons, as only 3. Most leading commercial snap bean varieties are resistant to smv, with only a restricted number showing susceptibility. The experiment was conducted in the screenhouse at the crop production pavilion, faculty of agriculture, university of ilorin. It infects mainly plants belonging to the family fabaceae but has.
The current status of the soybeansoybean mosaic virus. It is important to detect any new virus or new outbreak of a virus in virginia crops, and assess its economic or biosecurity significance. Soybean mosaic virus smv is seed and aphid transmitted and can cause significant reductions in yield and seed quality in soybean glycine max. Pdf bean yellow mosaic virus in soybean from argentina. Aphids feed on infected plants and transmit smv to healthy plants seasonlong. Quebra da resistencia em pimentao contra o pepper yellow mosaic virus. The current status of the soybeansoybean mosaic virus smv. Control of virus diseases in soybeans sciencedirect. Plantation, another virus, turnip mosaic virus tumv was identified from dames rocket hesperis matronalis l. The control of plant viruses relies heavily on phytosanitary applications, insect vector control, and the use of cultivars resistant to specific plant viruses. Section of plant pathology and plantmicrobe biology, school of integrative plant science, cornell university, new york state agricultural experiment station, geneva, ny 14456. The virus had a particle morphology and incubation period similar to those of other smv isolates but differed from them in the leaf symptoms it caused when infecting soybean and n. Transgenic soybean glycine max plants expressing soybean mosaic virus smv helper componentprotease hcpro showed altered vegetative and reproductive phenotypes and responses to smv.
Smv belongs to potyvirus and causes typical symptoms such as mild mosaic. Sometimes only part of the plant is affected with normal fruit maturing on the uninfected branches. Detection, diversity, and management of soybean mosaic virus. Virus diseases of beans fact sheet cornell university. Symptoms of soybean viruses include a mosaic pattern of light greenyellow and dark patches, distorted and wrinkled leaves, mosaic symptoms caused by soybean mosaic virus. Soybean mosaic virus smv smv is introduced into soybean fields with infected seed. The roles in seed and aphid transmission of selected smvencoded proteins were investigated by constructing mutants in and chimeric recombinants between smv 4 efficiently aphid and seed transmitted. Effect of different strains of soybean mosaic virus on growth, maturity, yield, seed mottling, and seed transmission in several soybean. Early infection is usually more severe and will produce plants. Most varieties will be stunted and have fewer pods.
Jul 01, 2014 fungicide recommendations are developed by the ncera7 national soybean disease committee, and recommendations are based on replicated research data collected from university trials. Soybean mosaic virus smv nia proteasemediated cleavage of. Molecular variability and genetic structure of the population of soybean mosaic virus based on the analysis of complete genome sequences. Ranga agriculture university, andhra pradesh, india corresponding author a b s t r a c t keywords article info. Soybean mosaic is caused by the potyvirus soybean mosaic virus. Fine mapping and candidate gene discovery of the soybean. Virus diseases constitute the most serious threat to soybean production in many tropical areas. First molecular evidence of soybean mosaic virus smv. Soybean mosaic virus is seedborne and ubiquitous in soybean, but has been effectively controlled through resistant cultivars. Reduced seed set can occur if plants are infected at the seedling stage. Infection of soybean seed parts and seed transmission g. To date, a few viruses, including bean pod mottle virus bpmv, cucumber mosaic virus cmv, and soybean mosaic virus smv, have been engineered as gene.
It causes symptoms similar to those produced by ryegrass mosaic virus, but is not transmitted by abacarus hystrix and does not react with antiserum to ryegrass mosaic virus mulligan, 1960. Gonsalves new york state agricultural experiment station, cornell university, geneva, ny 14456 some cultivars of phaseolus vulgaris l. Soybean mosaic virus smv and alfalfa mosaic virus amv are virus diseases of soybean that are transmitted by aphids as they feed on plant sap. Effects of cowpea mottle virus and cucumber mosaic virus. Several viruses can cause devastating diseases in domestic apple malus x. Role of soybean mosaic virusencoded proteins in seed and. Coinfection of both viruses increased the yield loss to as high as 86% and increase the rate of smv seed transmission. Furthermore, smv often infects soybeans with other viruses such as bean pod mottle virus bpmv, alfalfa mosaic virus amv and tobacco ringspot virus trsv wang, 2009.
The mosaic pattern in these soybean leaves is due to infection by bean pod mottle virus. Panjabrao deshmukh krishi vidyapeeth, akola, ms 444104, india corresponding author a b s t r a c t inoculum was prepared. Identified and released virus resistant germplasm, and mapped virus resistance genes including for maize chlorotic dwarf virus, maize dwarf mosaic virus, and sugarcane mosaic virus. Symptom severity is dependent upon the interaction between the soybean cultivar and virus strain. Soybean mosaic virus helper componentprotease alters leaf morphology and reduces seed production in transgenic soybean plants. Turnip mosaic virus tobacco etch virus soybean mosaic virus.
Over 30 species of aphid including aphis glycines, the soybean aphid, transmit the virus particle. Oct 30, 2014 the occurrence of soybean mosaic virus smv in soybean grown in midhill condition of meghalaya, india was confirmed by symptomatology, transmission electron microscopy, reverse transcriptionpolymerase chain reaction rtpcr and partial characterization of cytoplasmic inclusion protein ci, nuclear inclusion protein and coat protein nibcp domains. Soybean mosaic virus an overview sciencedirect topics. Aphid control is vital to control of bean mosaic virus.
Dissecting soybean defense pathways using virusinduced. Effects of cowpea mottle virus and cucumber mosaic virus on. Gene networks controlling phakopsora pachyrhizisoybean interactions. Bean yellow mosaic virus has a wide host range, is easily transmissible mechanically, and by more than 20 species of aphids in a nonpersistent manner. Soybean mosaic virus smv occurs in all soybeangrowing areas in the world and causes huge losses in soybean yields and seed quality. Mosaic virus overwinters on perennial weeds and is spread by insects that feed on them. Seed discoloration from soybean mosaic virus seed discoloration from soybean mosaic virus. Check the underside of the leaves for aphids and, if found, treat immediately with an insecticidal soap or neem oil.
Characterization of a soybean mosaic virus variant causing. Variation of cellfree translation profiles among pathogenic strains of soybean mosaic virus. Silencing of random cdnas encoded by the soybean genome will be carried out in an effort to identify novel components of the soybean. Smv is the cause of soybean mosaic disease that occurs in all the soybean productions areas of the word. Soybean aphid has been shown to transmit this virus. Managing white mold in soybean wisconsin field crops pathology. To date, a few viruses, including bean pod mottle virus bpmv, cucumber mosaic virus cmv, and soybean mosaic virus smv, have been engineered as gene delivery vectors for systemic expression. Cucumber mosaic virus in tomatoes is evidenced by stunted, yet bushy, growth. Ponceau s solution staining was included as a control to show equal loading of protein samples.
You can try covering your plants with a floating row cover or aluminum foil mulches to prevent these insects from infecting your plants. The virus is important on long bean, especially when infection comes from planting diseased seed. Soybean virus effort was increased on bpmv, found to be highly prevalent on the eastern shore and in two counties on the northern neck of virginia. Bean pod mottle virus wisconsin field crops pathology.
Soybean mosaic virus smv is a member of the plant virus genus potyvirus family potyviridae. It is named after its symptoms that were first present on apples. Soybean mosaic virus smv induces symptoms closely resembling those caused by bcmv. Project methods soybean orthologs of known arabidopsis defensesignaling components will be silenced using virus induced gene silencing and the effect of their silencing on the soybean resistance responses to the various pathogens will be studied.
Pdkv, akola, 444104, maharashtra, india 2department of plant pathology, achaya n. Bean yellow mosa ic virus h as a wide host r ange, is easily transmissible mechanically, a nd by more than 20 species of aphids in a nonpersistent manner. Cucumber mosaic virus cmv is one of the most common plant viruses, causing yellow mottling, distorted leaves and stunted growth in a wide range of garden plants, not just cucumbers. With soybeans, alfalfa mosaic virus amv, bean pod mottle virus bpmv, and soybean mosaic virus smv can substantially reduce yield, but also cause seed coat mottling, which directly impacts the sales of high quality food grade soybeans, one of ohios top export crops.